"Five people were shot dead today in targeted killing incidents," senior police official Fayyaz Leghari told AFP.
Late Monday the death toll had stood at 32 after three days of murders, he said. Police were searching for attackers in eastern and southern parts of city where the violence took place, Leghari said.
Sharmila Farooqi, an advisor in the provincial government of Sindh, of which Karachi is the capital, told AFP: "Some 50 people were injured and 60 people have been arrested in connection with the targeted killings" since Saturday.
She was able to confirm 36 deaths, she said.
Most of Karachi was open as normal, although witnesses reported violence in the volatile and low-income Layari neighbourhood.
Interior Minister Rehman Malik held talks in Karachi on Monday on how to end the violence, which has killed supporters of local coalition partners the Muttahida Qaumi Movement (MQM) and the Awami National Party (ANP).
Both parties accuse each other of killing their supporters, fanning political tensions within Karachi that reverberate to the capital Islamabad, where both factions are also members of the ruling federal coalition.
Karachi is plagued by ethnic and sectarian killings, crime and kidnappings.
A wave of violence that followed the August killing of MQM provincial lawmaker Raza Haider claimed 85 lives in the teeming city of 16 million.
MQM's Saifuddin Khalid easily won a by-election on Sunday to replace Haider in an election that ANP boycotted.
A founding member of the MQM, Imran Farooq, who was living in exile in Britain, was also brutally murdered outside his north London home in September.
The MQM represents the Urdu-speaking majority in Karachi, while the ANP is focused on Pashtuns who migrated from the northwest.
The government has not released exact figures, but rights groups say more than 260 targeted killings were reported in Karachi during the first six months of this year, compared with 156 during the same period in 2009.